Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)

Authors
Surenciski, Mauro Rodrigo; Dematteis, Massimiliano; Flachsland, Eduardo Alberto
Publication Year
2007
Language
English
Format
article
Status
Published version
Description
Cyrtopodium hatschbachii is a recently-described orchid species distributed in norteastern Argentina. Natural populations of this species require conservation because they have a very restricted size and distribution and are in danger of destruction. Cryopreservation in liquid nitrogen is the most commonly-applied technique for ex situ conservation of plant genetic resources, but genetic stability after cryopreservation is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after cold storage. We analyzed plants of C. hatschbachii cytogenetically in order to determinate their stability level. The plants from cryopreserved encapsulated seeds were stable at chromosome and phenotypic level, but showed more limited condensation of the chromatin during the first stages of their development. The chromosome number and karyotype analysis of C. hatschbachii is presented for the first time.
Fil: Surenciski, Mauro Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Dematteis, Massimiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Subject
cryopreservation
Cyrtopodium
encapsulation-dehydration
karyotype
orchid germplasm
Ciencias de las Plantas, Botánica
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/45137