Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae).

Authors
Sosa, María de las Mercedes; Angulo, Maria Betiana; Greppi, Julián Alejandro; Bugallo, Verónica
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Cytogenetic characterization and determination of DNA content by flow cytometry of five species of MecardoniaRuiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for M. flagellaris(Chamisso & Schlechtendal, 1827) (2n = 22), M. grandiflora(Bentham) Pennell, 1946 (2n = 22), M. kamogawaeGreppi & Hagiwara, 2011 (2n = 66), and Mecardoniasp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardoniaand appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus.
Fil: Sosa, María de las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Angulo, Maria Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Greppi, Julián Alejandro. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Bugallo, Verónica. Instituto Nacional de Tecnología Agropecuaria; Argentina
Subject
DNA amount
chromosome number
Gratiolae
DNA content
flow cytometry
polyploidy
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/30463