Arbuscular mycorrhizal fungal diversity in rhizosphere spores versus roots of an endangered endemic tree from Argentina: Is fungal diversity similar among forest disturbance types?

Authors
Soteras, María Florencia; Moreira, Bruno Coutinho; Grilli, Gabriel; Pastor, Nicolás; Mendes, Flávia Carneiro; Carvalho, Daniela Ruela de; Renison, Daniel; Kasuya, Maria Catarina Megumi; Souza, Francisco Adriano de; Becerra, Alejandra Gabriela
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
The aim of this study was to compare the arbuscular mycorrhizalfungal (AMF) community of the rhizosphere and inside the roots of theperennial Polylepis australis tree. Three forest types differing in theirstructural complexity due to anthropogenic disturbances were chosen atthree different sites at the high mountains of central Argentina.Rhizosphere spores and P. australis roots of four randomly selected treeswere isolated from 36 soil samples, DNA was extracted and the 18S rDNAfragments were amplified by nested-PCR. The products were analyzed byDGGE and the bands were excised for sequencing. In total, 36 OTUs weredefined from 56 DGGE bands successfully sequenced. Forest disturbancetypes showed similar communities of AMF, as rhizosphere spores and withinthe roots of P. australis. However, DGGE clustering showed mainlydifferences between rhizosphere spores and root-colonizing AMF. Membersof Glomeraceae, Pacisporaceae, Acaulosporaceae and Gigasporaceae wereshown in rhizosphere spore samples. Root samples showed only members ofAcaulosporaceae and Gigasporaceae, which might be complementary in termsof soil resources exploration. The prevalence of the root system withtheir community of symbionts might explain the resilience of AMF soilcommunities to forests structural changes. This study presents evidenceof a possible preference in the AMF- P. australis interaction.
Fil: Soteras, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Moreira, Bruno Coutinho. Universidade Federal de Viçosa; Brasil
Fil: Grilli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Pastor, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Mendes, Flávia Carneiro. Universidade Federal de Viçosa; Brasil
Fil: Carvalho, Daniela Ruela de. Universidade Federal de Viçosa; Brasil
Fil: Renison, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Kasuya, Maria Catarina Megumi. Universidade Federal de Viçosa; Brasil
Fil: Souza, Francisco Adriano de. Núcleo de Biologia Aplicada; Brasil
Fil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Subject
GLOMEROMYCOTA
SYMBIONTS
SOIL
OTU
POLYLEPIS AUSTRALIS
SOUTH AMERICA
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/23268