Histologic effect of a postnatal slow-release GnRH agonist on feline gonads

Authors
Carranza Martin, Ana Cristina; Faya, Marcela Inés; Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Gobello, María Cristina
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
In postnatal domestic cats, GnRH agonists suppressed fecal concentrations of sexual steroids and delayed puberty. The aim of this study was to describe the gross and microscopic morphometric effects of a single administration of the GnRH agonist, deslorelin acetate, on the gonads of postnatally treated cats. Twenty-seven postnatal male (n=14) and female (n=13) kittens were randomly assigned to one of the following treatment groups within the first 24hours of birth: deslorelin acetate (1.6mg, subcutaneous; DA, n=16) or control that remained untreated (CO, n=11) and spayed or castrated immediately after the onset of puberty. After surgical removal, the gonads were gross and histologically assessed. Sertoli cells also were examined immunohistochemically. Comparisons between the treatments were carried out by the Student t test. Gross gonadal wet weight and volume as well as gonadosomatic index were significantly lower in the DA than those in the CO males; these same parameters were not different in females. Primordial (461.4+3.0 vs. 1074.3+117.5; P<0.01), primary (59.1+13.5 vs. 165.4+24.6; P<0.01), and secondary (17.5+2.6 vs. 31.17+8.1; P<0.05) follicles per mm2 were decreased in DA than in CO gonads. Epididymal sperm motility and morphology were normal in all but two DA cats that had too few sperm to be evaluated. Germinal epithelial height (μm; 39.68+0.92 vs. 72.7+1.2; P<0.01) and most of their cellular components as well as the Sertoli (cm3; 0.1+0.02 vs. 0.24+0.05; P<0.01) cells were diminished in the DA cats. Gonadotropin-releasing hormone agonist endocrine disruption during the neonatal critical reproductive time window may have a potential as a contraceptive agent in domestic felids.
Fil: Carranza Martin, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faya, Marcela Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Patricia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Subject
FELINE
GNRH AGONIST
GONAD
NEONATAL
Otras Ciencias Veterinarias
Ciencias Veterinarias
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/53606