A Fungal Effector With Host Nuclear Localization and DNA-Binding Properties Is Required for Maize Anthracnose Development

Authors
Vargas, Walter Alberto; Sanz Martín, José M.; Rech, Gabriel E.; Armijos Jaramillo, Vinicio D.; Rivera Rodriguez, Lina Patricia; Echeverria, María de Las Mercedes; Díaz Mínguez, José M.; Thon, Michael R.; Sukno, Serenella A.
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Plant pathogens have the capacity to manipulate the host immune system through the secretion of effectors. We identified 27 putative effector proteins encoded in the genome of the maize anthracnose pathogen Colletotrichum graminicola that are likely to target the host’s nucleus, as they simultaneously contain sequence signatures for secretion and nuclear localization. We functionally characterized one protein, identified as CgEP1. This protein is synthesized during the early stages of disease development and is necessary for anthracnose development in maize leaves, stems, and roots. Genetic, molecular, and biochemical studies confirmed that this effector targets the host’s nucleus and defines a novel class of double-stranded DNA-binding protein. We show that CgEP1 arose from a gene duplication in an ancestor of a lineage of monocot-infecting Colletotrichum spp. and has undergone an intense evolution process, with evidence for episodes of positive selection. We detected CgEP1 homologs in several species of a grass-infecting lineage of Colletotrichum spp., suggesting that its function may be conserved across a large number of anthracnose pathogens. Our results demonstrate that effectors targeted to the host nucleus may be key elements for disease development and aid in the understanding of the genetic basis of anthracnose development in maize plants.
Fil: Vargas, Walter Alberto. Universidad de Salamanca; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanz Martín, José M.. Universidad de Salamanca; España
Fil: Rech, Gabriel E.. Universidad de Salamanca; España
Fil: Armijos Jaramillo, Vinicio D.. Universidad de Salamanca; España
Fil: Rivera Rodriguez, Lina Patricia. Universidad de Salamanca; España
Fil: Echeverria, María de Las Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Díaz Mínguez, José M.. Universidad de Salamanca; España
Fil: Thon, Michael R.. Universidad de Salamanca; España
Fil: Sukno, Serenella A.. Universidad de Salamanca; España
Subject
MAIZE
ANTHRACNOSE
COLLETOTRICHUM GRAMINICOLA
NUCLEAR EFFECTOR
Agricultura
Agricultura, Silvicultura y Pesca
CIENCIAS AGRÍCOLAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/43702