A fast chromatographic method for determination of daidzein and genistein in spiked water river samples using multivariate curve resolution

Authors
Moreira, Edilene D.T.; Pinto, Licarion; Gomes, Adriano A.; Goicoechea, Hector Casimiro; Araujo, Mario C.U.
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
This work reports the development of a fast chromatography methodology for quantitation of two phytoestrogens: daidzein (DAI) and genistein (GEN), in river water samples. The proposed method is based on High performance liquid chromatography-diode array detection (HPLC-DAD) data, and multivariate curve resolution-alternative least square (MCR-ALS) second-order calibration. Initially, the method was evaluated analysing a synthetic validation set, prepared based on a Taguchi design. Subsequently, the method was applied to predict the concentration of the phytoestrogens in river water samples, previously pre-processed by solid phase extraction (SPE). By implementation of the present methodology, a 50 % of reduction in operation time was achieved (from 7.00 to 3.25 min) when compared with previous work in literature. Precision were achieved even in the presence non modelled constituents and strong background. Thus, it has shown that the proposed method to be abecome in a rapid and robust alternative for the quantitation of studied phytoestrogens.
Fil: Moreira, Edilene D.T.. Universidade Federal da Paraíba. Departamento de Química. Laboratorio de Automacao e instrumentacao em quimica analitica e quimiometria; Brasil
Fil: Pinto, Licarion. Universidade Federal da Paraíba. Departamento de Química. Laboratorio de Automacao e instrumentacao em quimica analitica e quimiometria; Brasil
Fil: Gomes, Adriano A.. Universidade Federal da Paraíba. Departamento de Química. Laboratorio de Automacao e instrumentacao em quimica analitica e quimiometria; Brasil
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Araujo, Mario C.U.. Universidade Federal da Paraíba. Departamento de Química. Laboratorio de Automacao e instrumentacao em quimica analitica e quimiometria; Brasil
Subject
emerging contaminants
liquid chromatography
multiway calibration
second order advantage
Química Analítica
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/9376