Protective effects of the synthetic cannabinoids CP55,940 and JWH-015 on rat brain mitochondria upon paraquat exposure

Authors
Velez Pardo, Carlos; Jimenez Del Rio, Marlene; Lores Arnaiz, Silvia; Bustamante, Juanita
Publication Year
2010
Language
English
Format
article
Status
Published version
Description
The effects of cannabinoids in mitochondria after acute oxidative stress insult are not fully established. We investigated the ability of CP55,940 and JWH-015 to scavenge reactive oxygen species and their effect on mitochondria permeability transition (MPT) in either A mitochondria-free superoxide anion generation system, intact rat brain mitochondria or in sub-mitochondrial particles (SMP) treated with paraquat (PQ). Oxygen consumption, mitochondrial membrane potential (δ φm) and MPT were determined as parameters of mitochondrial function. It is found that both cannabinoids effectively attenuate mitochondrial damage against PQ-induced oxi-dative stress by scavenging anion superoxide radical (O2-) and hydrogen peroxide (H2O2), maintaining φm and by avoiding Ca2+-induced mitochondrial swelling. Understanding the mechanistic action of cannabinoids on mitochondria might provide new insights into more effective therapeutic approaches for oxidative stress related disorders.
Fil: Velez Pardo, Carlos. Universidad de Antioquia; Colombia
Fil: Jimenez Del Rio, Marlene. Universidad de Antioquia; Colombia
Fil: Lores Arnaiz, Silvia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustamante, Juanita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Subject
CANNABINOIDS
CP55,940
JWH-015 MITOCHONDRIA
OXIDATIVE STRESS
PARAQUAT
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/67604