(–)-Epicatechin reduces blood pressure and improves vasorelaxation in spontaneously hypertensive rats by NO-mediated mechanism

Authors
Galleano, Mónica Liliana; Bernatova, Iveta; Puzserova, Angelika; Balis, Peter; Sestakova, Natalia; Pechanova, Olga; Fraga, César Guillermo
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Studies in humans have found consumption of certain flavanoid-containing foods to be associated with improvement in endothelial function and with reduction of blood pressure (BP). (-)-Epicatechin is a compound representative of the flavanols (a subfamily of flavonoids), abundant in cocoa seeds, which is preserved during the industrialization process to chocolate. The antihypertensive effect of dietary (-)-epicatechin was investigated on spontaneously hypertensive rats (SHR). Consumption of (-)-epicatechin-supplemented diet (3 g (-)-epicatechin/kg diet) decreased blood pressu re (BP) in SHR by 27 and 23 mm of Hg on days 2 and 6, respectively. On day 6,a 173% increase of nitric oxide (NO) synthase activity was observed in the aorta of EPI-SHR as compared to non-supplemented SHR (p<0.05). Responses to acetylcholine (ACh) were then examined in femoral arteries in the absence and the presence of with L-NAME, a non-selective NOS inhibitor, to assess the Ach-mediated relaxation ascribed to NO-dependent and independent mechanisms Acetylcholine-induced endothelium-dependent relaxation in the femoral artery was significantly higher in EPI-SHR than in SHR, with a predominance of the NO-dependent component of this relaxation. The endothelium-independent relaxation, assayed by using the NO donor sodium nitroprusside (SNP), resulted non significant different in the three experimental groups, demonstrating an unaffected function of vascular smooth muscle cells. These results give further support to the concept that (-)-epicatechin can modulate BP in hypertension by increasing NO levels in the vasculature.
Fil: Galleano, Mónica Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Bernatova, Iveta. Slovak Academy of Sciences; Eslovaquia
Fil: Puzserova, Angelika. Slovak Academy of Sciences; Eslovaquia
Fil: Balis, Peter. Slovak Academy of Sciences; Eslovaquia
Fil: Sestakova, Natalia. Slovak Academy of Sciences; Eslovaquia
Fil: Pechanova, Olga. Slovak Academy of Sciences; Eslovaquia
Fil: Fraga, César Guillermo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Subject
FLAVONOIDS
HYPERTENSION
NITRIC OXIDE
PHYTONUTRIENTS
COCOA
CHOCOLATE
ENDOTHELIAL DYSFUNCTION
Nutrición, Dietética
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/21283