A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus

Authors
Garcia Guizzo, Melina; Parizi, Luís Fernando; Nunes, Rodrigo Dutra; Schama, Renata; Albano, Rodolpho M.; Tirloni, Lucas; Oldiges, Daiane Patrícia; Pilz Vieira, Ricardo; Cruz Oliveira, Wanderson Henrique; De Souza Leite, Milane; Gonzales, Sergio A.; Farber, Marisa Diana; Martins, Orlando; Da Silva Vaz, Itabajara; Oliveira, Pedro L.
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro; Brasil
Fil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; Brasil
Fil: Albano, Rodolpho M.. Universidade do Estado do Rio de Janeiro; Brasil
Fil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Gonzales, Sergio A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Martins, Orlando. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Subject
APPLIED MICROBIOLOGY
SYMBIOSIS
METAGENOMICS
TICKS
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/41664