Preservation of Solanum pimpinellifolium genomic fragments in recombinant genotypes increase tomato fruit quality

Authors
Mahuad, Sabina Lara; Pratta, Guillermo Raúl; Rodríguez, Gustavo Rubén; Zorzoli, Roxana; Picardi, Liliana Amelia
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Five recombinant inbred lines obtained from the F2 generation of an interspecific cross between cultivar, Caimanta (Cai, Solanum lycopersicum) and wild accession, LA722 (P, S. pimpinellifolium) were crossed to obtain the second cycle hybrids (SCH). Eleven fruit quality traits were assessed in evaluating phenotypic variability among genotypes P, Cai, F1 (Cai × P), five RILs, and 10 SCH. One of the five recombinant inbred lines and three SCH had higher values than P, as the best geno- type for shelf life. Sequence-related amplified polymorphism was used as the molecular method for detecting polymorphism among these 18 genotypes. The percentage of polymorphism in RILs and SCH was 61% and 66% respectively. Moreover, some bands detected in P were present in SCH. Several multivariate analyses were performed to find agreement between the phenotypic variability observed for fruit quality traits and the polymorphism obtained from sequence-related amplified poly- morphism markers. A general Procrustes analysis estimated that there was a consensus proportion of 75% between phenotypic and molecular data. There was considerable preservation of some bands from the wild genotype, which could increase the variability in fruit quality traits in populations where the genetic diversity is limited.
Fil: Mahuad, Sabina Lara. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina;
Fil: Pratta, Guillermo Raúl. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina;
Fil: Rodríguez, Gustavo Rubén. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Cordoba. Instituto de Humanidades; Argentina;
Fil: Zorzoli, Roxana. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Fil: Picardi, Liliana Amelia. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Subject
PLANT GENETIC RESOURCES
SECOND CYCLE HIBRYDS
SRAP
GENERALISED PROCRUSTER ANALYSIS
MOLECULAR MARKERS
Ciencias Agrícolas
Agricultura, Silvicultura y Pesca
Horticultura, Viticultura
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/926