Biokinetics and tissue response to ultrananocrystalline diamond nanoparticles employed as coating for biomedical devices

Authors
Tasat, Deborah Ruth; Bruno, Marcos E.; Domingo, Mariela Gisele; Gurman, Pablo; Auciello, Orlando; Paparella, María Luisa; Evelson, Pablo Andrés; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Although Ultrananocrystalline diamond (UNCD) has been proposed as a coating material for titanium biomedical implants, the biological effects and toxicity of UNCD particles that could eventually detach have not been studied to date. The biokinetics and biological effects of UNCD compared to titanium dioxide (TiO2) nanoparticles was evaluated in vivo using Wistar rats (n = 30) i.p. injected with TiO2, UNCD or saline solution. After 6 months, blood, lung, liver, and kidney samples were histologically analyzed. Oxidative damage by membrane lipidperoxidation (thiobarbituric acid reactive substances-TBARS), generation of reactive oxygen species (superoxide anion- O2 -), and antioxidant enzymes (superoxide dismutase-SOD, catalase-CAT) was evaluated in lung and liver. Histologic observation showed agglomerates of TiO2 or UNCD in the parenchyma of the studied organs, though there were fewer UNCD than TiO2 deposits. In addition, TiO2 caused areas compatibles with foci of necrosis in the liver and renal hyaline cylinders. Regarding UNCD, no membrane damage (TBARS) or mobilization of enzymatic antioxidants was observed either in lung or liver samples. No variations in O2 - generation were observed in lung (Co: 35.1 ± 4.02 vs. UNCD: 48 ± 9.1, p > 0.05). Conversely, TiO2 exposure caused production of O2 - in alveolar macrophages and consumption of catalase (p < 0.05). The studied parameters suggest that UNCD caused neither biochemical nor histological alterations, and therefore may prove useful as a surface coating for biomedical implants.
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bruno, Marcos E.. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Domingo, Mariela Gisele. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Interuniversitario Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gurman, Pablo. University of Texas; Estados Unidos
Fil: Auciello, Orlando. University of Texas; Estados Unidos
Fil: Paparella, María Luisa. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Evelson, Pablo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Subject
BIOKINETICS
OXIDATIVE METABOLISM
TITANIUM DIOXIDE
ULTRANANOCRYSTALLINE DIAMOND NANOPARTICLES
Salud Ocupacional
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/48643