Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol

Authors
Regiart, Daniel Matias Gaston; Seia, Marco Abel; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pg mL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Subject
ELECTROCHEMICAL
IMMUNOSENSOR
NANOCOMPOSITE
α-ZEARALANOL
BOVINE SERUM
Química Analítica
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/5786