Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior

Authors
Viñarta, Silvana Carolina; Delgado, Osvaldo Daniel; Castellanos de Figueroa, Lucia Ines; Fariña, Julia Inés
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Aqueous solutions (0.2%, w/v) of scleroglucans from Sclerotium rolfsii ATCC 201126 from different cultivation time or purification protocol (EPS I, EPS II, EPSi) as well as a commercial scleroglucan (LSCL) exhibited different sensitivity against thermal (65, 95 and 150 ◦C), ultrasonic (1, 5 and 10 min; 20% amplitude) or alkaline (0.01-0.2 N NaOH) treatments. Scleroglucan triple helix usually showed signs of denaturation at 150 ◦C or with 0.2 NaOH with a pronounced decrease in apparent viscosity and loss of pseudoplastic behavior. Differences in sensitivity could be noted depending on the scleroglucan sample, which may be likely related to polysaccharide conformational features, and these latter to production and/or downstream processing conditions. Transmission electronmicroscopy showed scleroglucan topologies in accordance with thermal and alkaline denaturation. Size exclusion chromatography of control scleroglucans revealed elution profiles compatible with macromolecular aggregates which tended to diminish or disappear as thermal, alkali or sonication treatments progressed. Scleroglucan granule dissolution process took ∼8-14 s, according to DIC light microscopy, and showed to be facilitated by addition of NaOH.
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina; Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina;
Fil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina;
Fil: Fariña, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina; Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina;
Subject
Scleroglucan
Thermal treatments
Alkaline treatments
Ultrasonic treatments
Físico-Química, Ciencia de los Polímeros, Electroquímica
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/1825