Metabolic engineering of lactic acid bacteria for the production of nutraceuticals

Authors
Hugenholtz, Jeroen; Sybesma, Wilbert; Groot, Masja Nierop; Wisselink, Wouter; Ladero, Victor; Burgess, Kay; van Sinderen, Douwe; Piard, Jean-Christophe; Eggink, Gerrit; Smid, Eddy J.; Savoy, Graciela; Sesma, Fernando Juan Manuel; Jansen, Tanja; Hols, Pascal; Kleerebezem, Michiel
Publication Year
2002
Language
English
Format
article
Status
Published version
Description
Lactic acid bacteria display a relatively simple and well-described metabolism where the sugar source is converted mainly to lactic acid. Here we will shortly describe metabolic engineering strategies on the level of sugar metabolism, that lead to either the efficient re-routing of the lactococcal sugar metabolism to nutritional end-products other than lactic acid such as L-alanine, several low-calorie sugars and oligosaccharides or to enhancement of sugar metabolism for complete removal of (undesirable) sugars from food materials. Moreover, we will review current metabolic engineering approaches that aim at increasing the flux through complex biosynthetic pathways, leading to the production of the B-vitamins folate and riboflavin. An overview of these metabolic engineering activities can be found on the website of the Nutra Cells 5th Framework EU-project (www.nutracells.com). Finally, the impact of the developments in the area of genomics and corresponding high-throughput technologies on nutraceutical production will be discussed.
Fil: Hugenholtz, Jeroen. Wageningen Centre for Food Sciences; Países Bajos. NIZO Food Research; Países Bajos
Fil: Sybesma, Wilbert. Wageningen Centre for Food Sciences; Países Bajos
Fil: Groot, Masja Nierop. Wageningen Centre for Food Sciences; Países Bajos
Fil: Wisselink, Wouter. Wageningen Centre for Food Sciences; Países Bajos. ATO-DLO; Países Bajos
Fil: Ladero, Victor. Université Catholique de Louvain, Louvain-la-neuve, Bel; Bélgica
Fil: Burgess, Kay. University College Cork; Irlanda
Fil: van Sinderen, Douwe. University College Cork; Irlanda
Fil: Piard, Jean-Christophe. Institut National de la Recherche Agronomique; Francia
Fil: Eggink, Gerrit. Wageningen Centre for Food Sciences; Países Bajos. ATO-DLO; Países Bajos
Fil: Smid, Eddy J.. NIZO Food Research; Países Bajos
Fil: Savoy, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Jansen, Tanja. NIZO Food Research; Países Bajos
Fil: Hols, Pascal. Université Catholique de Louvain; Bélgica
Fil: Kleerebezem, Michiel. Wageningen Centre for Food Sciences; Países Bajos. NIZO Food Research; Países Bajos
Subject
LACTIC ACID BACTERIA
NUTRACEUTICALS
FUNCTIONAL FOODS
FOLATE
GALACTOSE
LACTOCOCCUS LACTIS
LOW-CALORIE SUGARS
MANNITOL
METABOLIC ENGINEERING
OLIGOSACCHARIDES
RIBOFLAVIN
SORBITOL
TAGATOSE
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/58654