Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet

Authors
Grilli, Diego Javier; Fliegerová, Katerina; Kopecný, Jan; Paez Lama, Sebastián Antonio; Egea, Angela Vanina; Sohaefer, Noelia; Pereyra, Celia; Ruiz, María Soledad; Sosa Escudero, Miguel Angel; Arenas, Graciela Nora; Mrázek, Jakub
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied animals. DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain-fed animals, but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fed animals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days after the incorporation of corn into the diet and significantly lower concentration of bacteria belonging to Actinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day 20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantly higher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fed animals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals. The three different culture-independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet.
Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad "Juan Agustín Maza". Facultad de Ciencias Veterinarias y Ambientales; Argentina
Fil: Fliegerová, Katerina. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Kopecný, Jan. Institute Of Animal Physiology And Genetics.; República Checa
Fil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Egea, Angela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Sohaefer, Noelia. Universidad "Juan Agustín Maza". Facultad de Ciencias Veterinarias y Ambientales; Argentina
Fil: Pereyra, Celia. Universidad "Juan Agustín Maza". Facultad de Ciencias Veterinarias y Ambientales; Argentina
Fil: Ruiz, María Soledad. Universidad "Juan Agustín Maza". Facultad de Ciencias Veterinarias y Ambientales; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Mrázek, Jakub. Institute Of Animal Physiology And Genetics.; República Checa
Subject
Creole goats
Rumen
Bacteria
PCR-DGGE
qPCR
NGS
Otras Ciencias Veterinarias
Ciencias Veterinarias
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/44845