Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany.

Authors
Moré, Gastón Andrés; Pantchev, Nikola; Herrmann, Daland C.; Globokar Vrhovec, Majda; Öfner, Sabine; Conraths, Franz J.; Schares, Gereon
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Sarcocystis spp. represent apicomplexan parasites. They usually have a heteroxenous life cycle. Around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. In recent years, large numbers of reptiles have been imported into Europe as pets and, as a consequence, animal welfare and species protection issues emerged. A sample of pooled feces from four confiscated green pythons (Morelia viridis) containing Sarcocystis spp. sporocysts was
investigated. These snakes were imported for the pet trade and declared as being captive-bred. Full length 18S rRNA genes were amplified, cloned into plasmids and sequenced. Two different Sarcocystis spp. sequences were identified and registered as Sarcocystis sp. from M. viridis in GenBank. Both showed a 95?97% sequence identity with the 18S rRNA gene of Sarcocystis singaporensis. Phylogenetic analysis positioned these sequences together with other Sarcocystis spp. from snakesand rodents as definitive and intermediate hosts (IH), respectively. Sequence data and also the results of clinical and parasitological examinations suggest that the snakeswere definitive hosts for Sarcocystis spp. that circulate in wild IH. Thus, it seems unlikely that the infected snakes had been legally bred. Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species.
Fil: Moré, Gastón Andrés. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Pantchev, Nikola. IDEXX Vet Med Lab; Alemania
Fil: Herrmann, Daland C.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Fil: Globokar Vrhovec, Majda. IDEXX Vet Med Lab; Alemania
Fil: Öfner, Sabine. Reptile Rescue Centre Munich; Alemania
Fil: Conraths, Franz J.. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Fil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania
Subject
SARCOCYSTIS SPP.
18S rRNA GENE
SEQUENCING
REPTILES
SPECIES PROTECTION
Otras Ciencias Veterinarias
Ciencias Veterinarias
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/31150