Formation and colloidal stability of ovalbumin-retinol nanocomplexes

Authors
Visentini, Flavia Fátima; Sponton, Osvaldo Ernesto; Perez, Adrián Alejandro; Santiago, Liliana G.
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
This work is aimed to obtain and characterize nanocomplexes formed by non-covalent attractive interactions between ovalbumin (OVA) and retinol (RET). In order to gain some ideas about the effect of OVA aggregation on RET complexation, OVA nanoparticles (OVAn) obtained by controlled heat treatment (85 °C, 5 min) were also evaluated. Nanocomplex formation was monitored by intrinsic and extrinsic fluorescence spectroscopy. Results suggest that OVA was more effective for binding RET than OVAn, possibly because aggregate formation could employ some hydrophobic patches needed for RET binding. Furthermore, nanocomplex colloidal stability was studied by means of absorbance (at 400 nm), particle size distribution (PSD) and ζ potential determinations. At pH values of common foods (4.0 and 7.0), nanocomplex colloidal stability mainly depended on aqueous phase behaviour of the proteins (OVA and OVAn). Finally, long-term photochemical stability against light and oxygen of RET nanocomplexes depended on solution pH and protein aggregation.
Fil: Visentini, Flavia Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Sponton, Osvaldo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Perez, Adrián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Santiago, Liliana G.. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Subject
HEAT-INDUCED NANOPARTICLE
NANOCOMPLEXES
OVALBUMIN
PHOTOCHEMICAL STABILITY
RETINOL
VEHICULIZATION
Nano-materiales
Nanotecnología
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/67920