Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells

Authors
Hosoya, Soichi; Villena, Julio Cesar; Shimazu, Tomoyuki; Tohno, Masanori; Fujie, Hitomi; Chiba, Eriko; Shimosato, Takeshi; Aso, Hisashi; Suda, Yoshihito; Kawai, Yasushi; Saito, Tadao; Alvarez, Gladis Susana; Ikegami, Shuji; Itoh, Hiroyuki; Kitazawa, Haruki
Publication Year
2011
Language
English
Format
article
Status
Published version
Description
This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
Fil: Hosoya, Soichi. Tohoku University. Food Immunology Group; Japón
Fil: Villena, Julio Cesar. Tohoku University. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Shimazu, Tomoyuki. Tohoku University. Food Immunology Group; Japón
Fil: Tohno, Masanori. Tohoku University. Food Immunology Group; Japón
Fil: Fujie, Hitomi. Tohoku University. Food Immunology Group; Japón
Fil: Chiba, Eriko. Tohoku University. Food Immunology Group; Japón
Fil: Shimosato, Takeshi. Tohoku University. Food Immunology Group; Japón
Fil: Aso, Hisashi. Tohoku University. Food Immunology Group; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food Agriculture; Japón
Fil: Kawai, Yasushi. Tohoku University. Food Immunology Group; Japón
Fil: Saito, Tadao. Tohoku University. Food Immunology Group; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina
Fil: Ikegami, Shuji. Meiji Dairies Corporation Japan; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation Japan; Japón
Fil: Kitazawa, Haruki. Tohoku University. Food Immunology Group; Japón
Subject
PORCINE INTESTINAL EPITHELIAL CELLS
INFLAMMATORY RESPONSE
IMMUNOBIOTICS
POLY(I:C)
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/54793