Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?

Authors
Szawarski, Nicolás; Quintana, Silvina; Levy, Eugenia; Lucia, Mariano; Abrahamovich, Alberto Horacio; Porrini, Martín Pablo; Brasesco, Maria Constanza; Negri, Pedro; Sarlo, Gabriel; Eguaras, Martin Javier; Maggi, Matías Daniel
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
The honey bee tracheal mite Acarapis woodi (Rennie) (Acari: Tarsonemidae) is an internal obligate parasite of the respiratory system of adult honey bees (Apis mellifera), which may be one of the factors causing the loss of bee colonies and the decrease in the beekeeping industry. In Argentina, the last prevalence study of A. woodi was in 1998, and therefore there is no current data on the prevalence and impact of the mite in colonies of A. mellifera in this country. For this reason, the objective of this study was to determine the prevalence of A. woodi in honey bee colonies of apiaries belonging to different geographic regions of Argentina (Northwest, Chaco, Mesopotamic, Pampean and Patagonic) using two different methods of diagnosis. One method was traditional microscopy, which is based on direct visualization of A. woodi in the prothoracic tracheas of bees, and the other technique was molecular analysis using real-time PCR with specific primers to amplify CO1 gene of A. woodi. To perform the analysis of prevalence, bees were collected during winter, because during this season, populations of A. woodi are more abundant in bee colonies. The results were negative for both methods of diagnosis, with prevalence equal to 0%. Considering that in recent years it has been reported an increased mortality in bee colonies in Argentina, we note that acarapisosis is not currently impacting on the disappearance of A. mellifera colonies in Argentina.
Fil: Szawarski, Nicolás. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie. Laboratorio de Biología Molecular; Argentina
Fil: Levy, Eugenia. Universidad Nacional de Mar del Plata; Argentina
Fil: Lucia, Mariano. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abrahamovich, Alberto Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Brasesco, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sarlo, Gabriel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Subject
ACARAPIS WOODI
APIS MELLIFERA
ARGENTINA
DISSECTION METHOD CLASSIC
REAL-TIME PCR
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/50212