Analysis of persistence during intracellular actin-based transport mediated by molecular motors

Autores
Pallavicini, C.; <div class="autor_fcen" id="2494">Despósito, M.A.</div>; <div class="autor_fcen" id="4951">Levi, V.</div>; <div class="autor_fcen" id="1190">Bruno, L.</div>
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
Versión publicada
Descripción
The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.
Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Phys. Conf. Ser. 2010;246
Materia
Anomalous diffusion
Cell cytoplasm
Critical time
Function of time
In-cell
Molecular motors
Spatio-temporal resolution
Time lag
Tracking techniques
Turning angles
Xenopus laevis
Cytology
Linear motors
Proteins
Nivel de accesibilidad
Acceso abierto
Licencia
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
snrd:HASHc1de61fa988dc3884f0132