Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis

Autores
Nikel, P.I.; <div class="autor_fcen" id="6704">Pettinari, M.J.</div>; <div class="autor_fcen" id="3442">Galvagno, M.A.</div>; <div class="autor_fcen" id="5666">Méndez, B.S.</div>
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
Versión publicada
Descripción
We assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h-1, whereas the deletion mutant needed several nutritional additives and snowed a specific growth rate of 0.56 ± 0.06 h-1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Appl. Environ. Microbiol. 2006;72(4):2614-2620
Materia
Biomass
Cell culture
Escherichia coli
Mutagenesis
Strain
Synthesis (chemical)
ArcA mutants
Leaky mutation
Microaerobiosis
Poly(3-hydroxybutyrate) synthesis
Organic polymers
poly(3 hydroxybutyric acid)
bacteriology
gene
mutagenicity
recombination
aerobic metabolism
allele
arcA gene
arcA2 gene
article
Azotobacter
Azotobacter FA8
bacterial gene
bacterial growth
bacterial strain
biomass
bioreactor
dry weight
Escherichia coli
gene deletion
gene mutation
nonhuman
pha gene
phenotype
synthesis
wild type
Aerobiosis
Bacterial Outer Membrane Proteins
Biomass
Culture Media
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Hydroxybutyrates
Mutation
Polyesters
Recombination, Genetic
Repressor Proteins
Arca
Azotobacter
Bacteria (microorganisms)
Escherichia coli
Nivel de accesibilidad
Acceso abierto
Licencia
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
snrd:HASHcc68ecff4e4aa4311f5c19