Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth

Authors
<div class="autor_fcen" id="6909">Prado-Acosta, M.</div>; <div class="autor_fcen" id="7641">Ruzal, S.M.</div>; <div class="autor_fcen" id="163">Allievi, M.C.</div>; <div class="autor_fcen" id="6396">Palomino, M.M.</div>; Rivas, C.S.
Publication Year
2010
Language
English
Format
article
Status
Published version
Description
We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Source
Appl. Environ. Microbiol. 2010;76(3):974-977
Subject
Bacterial growth
Cell walls
Gram-positive bacterium
Hydrolases
Lactobacillus acidophilus
Proton-motive forces
S-layers
Salmonella enterica
Staphylococcus aureus
Surface layers
Synergistic effect
Bacillus cereus
Bacteriology
Cell membranes
Cytology
Antibiotics
antiinfective agent
food preservative
n acetylmuramoylalanine amidase
nisin
polylysine
surfactant
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
article
Bacillus cereus
bacterial count
bacterial gene
cell membrane
cell wall
drug effect
drug potentiation
enzymology
food control
Gram positive bacterium
growth, development and aging
heat
Lactobacillus acidophilus
microbiological examination
permeability
Staphylococcus aureus
Anti-Bacterial Agents
Bacillus cereus
Cell Membrane
Cell Wall
Colony Count, Microbial
Drug Synergism
Food Microbiology
Food Preservatives
Genes, Bacterial
Gram-Positive Bacteria
Hot Temperature
Lactobacillus acidophilus
Microbial Sensitivity Tests
N-Acetylmuramoyl-L-alanine Amidase
Nisin
Permeability
Polylysine
Staphylococcus aureus
Surface-Active Agents
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
Access level
Open access
License
http://creativecommons.org/licenses/by/2.5/ar
Repository
Biblioteca Digital (UBA-FCEN)
Institution
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identifier
snrd:HASH018db9874e099ea683ad9677