Elimination of D-lactate synthesis increases poly(3-hydroxybutyrate) and ethanol synthesis from glycerol and affects cofactor distribution in recombinant Escherichia coli

Autores
Nikel, P.I.; Giordano, A.M.; <div class="autor_fcen" id="2305">De Almeida, A.</div>; Godoy, M.S.; <div class="autor_fcen" id="6704">Pettinari, M.J.</div>
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
Versión publicada
Descripción
The effect of eliminating D-lactate synthesis in poly(3-hydroxybutyrate) (PHB)-accumulating recombinant Escherichia coli (K24K) was analyzed using glycerol as a substrate. K24KL, an ldhA derivative, produced more biomass and had altered carbon partitioning among the metabolic products, probably due to the increased availability of carbon precursors and reducing power. This resulted in a significant increase of PHB and ethanol synthesis and a decrease in acetate production. Cofactor measurements revealed that cultures of K24K and K24KL had a high intracellular NADPH content and that the NADPH/NADP+ ratio was higher than the NADH/NAD+ ratio. The ldhA mutation affected cofactor distribution, resulting in a more reduced intracellular state, mainly due to a further increase in NADPH/NADP+. In 60-h fed-batch cultures, K24KL reached 41.9 g · liter-1 biomass and accumulated PHB up to 63% ± 1% (wt/wt), with a PHB yield on glycerol of 0.41 ± 0.03 g · g-1, the highest reported using this substrate. © 2010, American Society for Microbiology.
Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Appl. Environ. Microbiol. 2010;76(22):7400-7406
Materia
Carbon partitioning
Carbon precursors
Cofactors
D-lactate
Fed-batch cultures
Metabolic products
Poly(3-hydroxybutyrate)
Recombinant Escherichia coli
Reducing power
Batch cell culture
Escherichia coli
Ethanol
Substrates
Glycerol
alcohol
carbon
glycerol
hydroxybutyric acid
lactate dehydrogenase
lactic acid
nicotinamide adenine dinucleotide
nicotinamide adenine dinucleotide phosphate
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
polyester
alcohol
biomass
ester
ethanol
fecal coliform
partitioning
recombination
article
biomass
bioreactor
chemistry
Escherichia coli
genetics
growth, development and aging
metabolism
time
Biomass
Bioreactors
Carbon
Escherichia coli
Ethanol
Glycerol
Hydroxybutyrates
Lactate Dehydrogenases
Lactic Acid
Metabolic Networks and Pathways
NAD
NADP
Polyesters
Time Factors
Escherichia coli
Nivel de accesibilidad
Acceso abierto
Licencia
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
snrd:HASH01008e6a7a803e0dcfffa447