Transport Properties of Melanosomes along Microtubules Interpreted by a Tug-of-War Model with Loose Mechanical Coupling

Autores
Bouzat, S.; <div class="autor_fcen" id="4951">Levi, V.</div>; <div class="autor_fcen" id="1190">Bruno, L.</div>
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
Versión publicada
Descripción
In this work, we explored theoretically the transport of organelles driven along microtubules by molecular motors of opposed polarities using a stochastic model that considers a Langevin dynamics for the cargo, independent cargo-motor linkers and stepping motion for the motors. It has been recently proposed that the stiffness of the motor plays an important role when multiple motors collectively transport a cargo. Therefore, we considered in our model the recently reported values for the stiffness of the cargo-motor linker determined in living cells (~0.01 pN/nm, [1]) which is significantly lower than the motor stiffness obtained in in vitro assays and used in previous studies. Our model could reproduce the multimodal velocity distributions and typical trajectory characteristics including the properties of the reversions in the overall direction of motion observed during melanosome transport along microtubules in Xenopus laevis melanophores. Moreover, we explored the contribution of the different motility states of the cargo-motor system to the different modes of the velocity distributions and could identify the microscopic mechanisms of transport leading to trajectories compatible with those observed in living cells. Finally, by changing the attachment and detachment rates, the model could reproduce the different velocity distributions observed during melanosome transport along microtubules in Xenopus laevis melanophores stimulated for aggregation and dispersion. Our analysis suggests that active tug-of-war processes with loose mechanical coupling can account for several aspects of cargo transport along microtubules in living cells. © 2012 Bouzat et al.
Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
PLoS ONE 2012;7(8)
Materia
molecular motor
article
cell adhesion
cell aggregation
cell motility
cell stimulation
cellular parameters
controlled study
dispersion
in vitro study
intracellular transport
mechanical coupling
melanophore
melanosome
microtubule
nonhuman
stochastic model
theoretical model
tug of war model
velocity
Xenopus laevis
Animals
Biological Transport
Biomechanics
Kinetics
Mechanical Processes
Melanosomes
Microtubules
Models, Biological
Molecular Motor Proteins
Movement
Stochastic Processes
Xenopus laevis
Xenopus laevis
Nivel de accesibilidad
Acceso abierto
Licencia
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
snrd:HASHb72b41bd1b2ddc82d4d39b