Abstract:

This report describes the draft genome sequence of Lactobacillus brevisTUCO-5E, a probiotic strain isolated from porcine maternal milk. The reads were generated by a whole-genome sequencing (WGS) strategy on an Illumina MiSeq sequencer and were assembled into contigs with a total estimated size of 2,461,089 bp. A total of 2,455 open reading frames (ORFs) were predicted, including 2,301 protein-coding sequences. The draft genome sequence of L. brevis TUCO-5E will be useful for further studies of specific genetic features and for understanding the mechanisms of its probiotic properties in the porcine host.

Author affiliation: Quilodrán-Vega, Sandra Rayén. Universidad de Concepción. Facultad de Medicina Veterinaria. Departamento de Medicina Preventiva y Patología; Chile

Author affiliation: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Author affiliation: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Author affiliation: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Author affiliation: Fonseca, Alexis. Universidad de Concepción. Departmento de Farmacología; Chile

Author affiliation: Salas-Burgos, Alexis. Universidad de Concepción. Departmento de Farmacología; Chile

Author affiliation: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón

Author affiliation: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina

Abstract:

In lactic acid bacteria, the synthesis of exopolysaccharides (EPS) has been associated with some favorable technological properties as well as health-promoting benefits. Research works have shown the potential of EPS produced by lactobacilli to differentially modulate immune responses. However, most studies were performed in immune cells and few works have concentrated in the immunomodulatory activities of EPS in non-immune cells such as intestinal epithelial cells. In addition, the cellular and molecular mechanisms involved in the immunoregulatory effects of EPS have not been studied in detail. In this work, we have performed a genomic characterization of Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and evaluated the immunomodulatory and antiviral properties of its acidic (APS) and neutral (NPS) EPS in porcine intestinal epithelial (PIE) cells. Whole genome sequencing allowed the analysis of the general features of L. delbrueckii TUA4408L genome as well as the characterization of its EPS genes. A typical EPS gene cluster was found in the TUA4408L genome consisting in five highly conserved genes epsA-E, and a variable region, which includes the genes for the polymerase wzy, the flippase wzx, and seven glycosyltransferases. In addition, we demonstrated here for the first time that L. delbrueckii TUA4408L and its EPS are able to improve the resistance of PIE cells against rotavirus infection by reducing viral replication and regulating inflammatory response. Moreover, studies in PIE cells demonstrated that the TUA4408L strain and its EPS differentially modulate the antiviral innate immune response triggered by the activation of Toll-like receptor 3 (TLR3). L. delbrueckii TUA4408L and its EPS are capable of increasing the activation of interferon regulatory factor (IRF)-3 and nuclear factor κB (NF-κB) signaling pathways leading to an improved expression of the antiviral factors interferon (IFN)-β, Myxovirus resistance gene A (MxA) and RNaseL.

Author affiliation: Kanmani, Paulraj. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón

Author affiliation: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón

Author affiliation: Kobayashi, Hisakazu. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón

Author affiliation: Hebert, Elvira Maria. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Author affiliation: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Author affiliation: Komatsu, Ryoya. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón

Author affiliation: Gatica, Brian. University of Concepcion. Departmento de Farmacología; Chile

Author affiliation: Miyazaki, Ayako. National Institute of Animal Health. Viral Diseases and Epidemiology Research Division; Japón

Author affiliation: Ikeda-Ohtsubo, Wakako. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón

Author affiliation: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture, and Environment; Japón

Author affiliation: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón

Author affiliation: Egusa, Shintaro. Marusan-Ai Co. Research & Development Division; Japón

Author affiliation: Mishima, Takashi. Mie University. Graduate School of Regional Innovation Studies; Japón

Author affiliation: Salas-Burgos, Alexis. University of Concepcion. Departmento de Farmacología; Chile

Author affiliation: Takahashi, Hideki. Tohoku University. Graduate School of Agricultural Science. Laboratory of Plant Pathology; Japón

Author affiliation: Villena, Julio Cesar. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Author affiliation: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group, Laboratory of Animal Products Chemistry; Japón