Abstract:
Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.
Inst. de Genética "Ewald A. Favret"- IGEAF
Author affiliation: Decima Oneto, Cecilia Andrea. INTA. Instituto de Genética "Ewald A. Favret"; Argentina
Author affiliation: Otegui, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Author affiliation: Baroli, Irene. INTA. Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Beznec, Ailin. INTA. Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Faccio, Paula Daniela. INTA. Instituto de Genética "Ewald A. Favret"; Argentina
Author affiliation: Bossio, Adrian EzequielINTA. Instituto de Genética "Ewald A. Favret"; Argentina
Author affiliation: Blumwald, Eduardo. University of California. Department Plant Sciences; Estados Unidos
Author affiliation: Lewi, Dalia Marcela. INTA. Instituto de Genética "Ewald A. Favret"; Argentina