Authors: Carnevale, Silvana; Malandrini, Jorge Bruno; Pantano, María Laura; Sawicki, Mirna; Soria, Claudia Cecilia; Kuo, Lein Hung; Kamenetzky, Laura; Astudillo, Osvaldo Germán; Velásquez, Jorge Néstor
Publication Date: 2016.
Language: English.
Abstract:
Fascioliasis is a zoonosis actually considered as a foodborne trematode disease priority by the World Health Organization. Our study presents three cases of F. hepatica infection diagnosed by direct, indirect and/or imaging diagnostic techniques, showing the need of the combined use of them. In order to overcome some difficulties of the presently available methods we show for the first time the application of molecular tools to improve human fascioliasis diagnosis by employing a PCR protocol based on a repetitive element as target sequence. In conclusion, diagnosis of human fascioliasis has to be carried out by the combination of diagnostic techniques that allow the detection of infection in different disease phases, different epidemiological situations and known/new transmission patterns in the actual scenario.
Author affiliation: Carnevale, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca; Argentina
Author affiliation: Pantano, María Laura. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Author affiliation: Sawicki, Mirna. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas ; Argentina
Author affiliation: Soria, Claudia Cecilia. Universidad Nacional de Catamarca; Argentina
Author affiliation: Kuo, Lein Hung. Provincia de Misiones. Hospital Samic Oberá; Argentina
Author affiliation: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Author affiliation: Astudillo, Osvaldo Germán. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Author affiliation: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas ; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Peralta, Regina Helena Saramago; Velásquez, Jorge Néstor; Cunha, Flavia de Souza; Pantano, María Laura; Sodré, Fernando Campos; Silva, Sidnei da; Astudillo, Osvaldo Germán; Peralta, José Mauro; Carnevale, Silvana
Publication Date: 2016.
Language: English.
Abstract:
The identification and characterisation of Cryptosporidiumgenotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity ofCryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays forCryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.
Author affiliation: Peralta, Regina Helena Saramago. Universidade Federal Fluminense; Brasil
Author affiliation: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Author affiliation: Cunha, Flavia de Souza. Universidade Federal Fluminense; Brasil
Author affiliation: Pantano, María Laura. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Author affiliation: Sodré, Fernando Campos. Universidade Federal Fluminense; Brasil
Author affiliation: Silva, Sidnei da. Fundación Oswaldo Cruz; Brasil
Author affiliation: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Author affiliation: Peralta, José Mauro. Universidade Federal do Rio de Janeiro; Brasil
Author affiliation: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Repository: CONICET Digital (CONICET). Consejo Nacional de Investigaciones Científicas y Técnicas
Authors: Carnevale, Silvana; Marta, Edgardo; Etchart, Cristina; Velásquez, Jorge N.; Astudillo, Osvaldo Germán; Chertcoff, Agustín; di Risio, Cecilia
Publication Date: 2010.
Language: Spanish.
Abstract:
El Cryptosporidium hominis (C hominis) es el protozoario que más comúnmente ha sido identificado en pacientes con SIDA. En este trabajo se describen las manifestaciones clínicas de un paciente con diarrea crónica y colangitis esclerosante asociada a SIDA. Los estudios por imágenes con ultrasonografía y colangiopancreatografía retrógrada endoscópica revelaron alteraciones en la vía biliar intra y extrahepática, idénticas a aquellas observadas en la colangitis esclerosante. Se detectó C hominis en duodeno y duodeno peripapilar por microscopía óptica y se confirmó por la reacción en cadena de la polimerasa anidada (nested PCR) en biopsias frescas seguida de análisis de polimorfismo de longitud de fragmentos de restricción. La infección con C hominis debería sospecharse en nuestro país en pacientes con inmunodeficiencia severa y colangitis esclerosante asociada a SIDA.
Cryptosporidium hominis (C hominis) is the most common protozoan parasite recognized in human patients with AIDS. We report the clinical features of a patient with chronic diarrhea and AIDS-related sclerosing cholangitis. The imaging studies with ultrasonography and endoscopic retrograde cholangiopancreatography disclosed intrahepatic and extrahepatic bile duct changes identical to those seen in sclerosing cholangitis. C hominis was detected in the duodenum and peripapillary duodenum by means of light microscopy and confirmed by nested polymerase chain reaction (PCR) amplification from fresh biopsy specimens followed by restriction length polymorphism analysis. C hominis infection should be suspected in our country in patients with advanced immunodeficiency and AIDS-related sclerosing cholangitis.
Author affiliation: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Author affiliation: Velásquez, Jorge Néstor. Hospital Municipal de Enfermedades Infecciosas "Dr Francisco J Muñiz"; Argentina.
Author affiliation: Marta, Edgardo. Hospital Municipal General de Agudos "Dr José María Penna"; Argentina.
Author affiliation: Astudillo, Osvaldo Germán. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Author affiliation: Etchart, Cristina. Hospital Municipal General de Agudos "Dr José María Penna"; Argentina.
Author affiliation: Chertcoff, Agustín. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Author affiliation: di Risio, Cecilia. Hospital Municipal General de Agudos "Dr José María Penna"; Argentina.
Repository: Sistema de Gestión del Conocimiento ANLIS MALBRÁN. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"